Inorganic phosphate (Pi) transport by rat Z310 choroid plexus (CP) epithelial cells suggests involvement of type III Na+‐Pi cotransporters (PiT1/2, slc20A1/2)
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Regulation of phosphate (Pi) transport and NaPi-III transporter (Pit-1) mRNA in rat osteoblasts.
In osteoblasts only the type III Na(+)-dependent phosphate (NaPi) transporter isoforms Pit-1 and Pit-2 have been identified. We tested the effects of extracellular Pi, Ca(2+) and IGF-I on Na(d)Pi transport and Pit-1 or Pit-2 mRNA expression in rat osteoblastic (PyMS) cells. The v(max) of Na(d)Pi transport was higher in cells kept in Pi-free, serum-free medium for 24 h than in controls at 1 mM P...
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During calcification of bone, large amounts of phosphate (P(i)) must be transported from the circulation to the osteoid. Likely candidates for osteoblast P(i) transport are the type II sodium-phosphate cotransporters NaPi-IIa and NaPi-IIb that facilitate transcellular P(i) flux in kidney and intestine, respectively. We have therefore determined the 'cotransporters' expression in osteoblast-like...
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A stable intraventricular milieu is crucial for maintaining normal neuronal function. The choroid plexus epithelium produces the cerebrospinal fluid and in doing so influences the chemical composition of the interstitial fluid of the brain. Here, we review the molecular pathways involved in transport of the electrolytes Na+, K+, Cl-, and HCO3(-)across the choroid plexus epithelium.
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The choroid plexus plays a wide range of roles in brain development, maturation, aging process, endocrine regulation, and pathogenesis of certain neurodegenerative diseases. To facilitate in vitro study, we have used a gene transfection technique to immortalize murine choroidal epithelial cells. A viral plasmid (pSV3neo) was inserted into the host genome of primary choroidal epithelia by calciu...
متن کاملبررسی کیفی و کمی بیان پروتئین آکواپورین1 در شبکه کوروئید رت نژاد سویتار
Abstract Background: Choroid plexus (CP) is a branched structure made up of a single layer of epithelial cells and blood capillaries, forming the blood-CSF-barrier. The CSF (cerebrospinal fluid) is mainly produced from the CP. Aquaporin1 (AQP1), water channels that are highly expressed on the apical side of the membrane in choroid plexus, have a major role in mediating water transport across th...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2011
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.25.1_supplement.650.4